D 10 mM citrate buffer, pH 6.0. To minimize non-specific staining, eacharea was handled with methanol containing 3 hydrogen peroxide for 3 min. Anti Bax and anti Bcl 2 antibodiesused at a dilution of 1:2000 and 1:1000, respectively in 0.05 M Tris buffered answer, pH 7.6 were put into the slide
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